保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
精製度Immunogen affinity purified
Our Abpromise guarantee covers the use of ab121254 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use buffer at pH 6 for antigen retrieval.|
機能Deubiquitinating enzyme which may play an important role during spermatogenesis.
配列類似性Belongs to the peptidase C19 family.
- Information by UniProt
- Deubiquitinating enzyme 42 antibody
- FLJ12697 antibody
- Ubiquitin carboxyl-terminal hydrolase 42 antibody
Immunofluorescent staining of Human cell line A-431 shows positivity in cytoplasm. Recommended concentration of ab121254 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemical staining of parffin embedded Human kidney, using 1/60 ab121254, shows strong nuclear cytoplasmic positivity in cells in tubules and cells in glomeruli.
ab121254 has not yet been referenced specifically in any publications.