Application
Western blot
Sample
Mouse Cell lysate - whole cell (platelet)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
platelet
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
Other product details
Incubation time
1 hour(s) and 0 minute(s)
Dilution
1/400
Secondary antibody
Dilution
1/15000
Name
Non-Abcam antibody was used: Odyssey
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 800CW
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 800CW
Detection
Detection method
Licor
Exposure
1 hour(s), 0 minute(s) and 0 second(s)
Bands
Specific: 74 kDa
Positive control
Human platelets
Additional data
Additional Notes
Human (lane 2) and mouse (lane 1) platelets were lysed using NP-40 lysis buffer and subjected to SDS-PAGE. A unique band of approximately 74 kDa was observed for both (green) using antibody ab68040). The blot was re-probed using an antibody against b-actin, as loading control (red).
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投稿 Feb 17 2015