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I would like to know if this cell line (U937) is an adherent type or not. I am cultivating these cells and would like to infect them with Leishmania parasite to determine their in vitro sensitivity to some antileishmanial drugs. If this cell line in not an adherent type, please advise on how could I make them to be adhered to the culture flasks or testing chamber slides.
Asked on May 18 2006
Thank you for your enquiry. U937 cells grow in suspension. To make them adhere, you can try using poly-lysine, fibronectin or collagen coated plates. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.
Abcam Scientific Support
Answered on May 23 2006