Application
Western blot
Sample
Human Cell lysate - whole cell (Human Fibroblast)
Loading amount
20 µg
Specification
Human Fibroblast
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris Nupage gel)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Dilution
1/2000
Incubation time
20 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: blocking buffer
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-Rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 680LT
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 680LT
Dilution
1/3000
Detection
Detection method
Licor
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 31 kDa Non-specific: 75, 43 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Jan 11 2013