Anti-Tyrosyl tRNA synthetase/TyrRS 抗体 [YAR5H08] (ab50961)
Key features and details
- Mouse monoclonal [YAR5H08] to Tyrosyl tRNA synthetase/TyrRS
- Suitable for: WB, IP, ICC
- Reacts with: Mouse, Human
- Isotype: IgG1
製品の概要
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製品名
Anti-Tyrosyl tRNA synthetase/TyrRS antibody [YAR5H08]
Tyrosyl tRNA synthetase/TyrRS 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [YAR5H08] to Tyrosyl tRNA synthetase/TyrRS -
由来種
Mouse -
アプリケーション
適用あり: WB, IP, ICCmore details -
種交差性
交差種: Mouse, Human -
免疫原
Recombinant fragment corresponding to Human Tyrosyl tRNA synthetase/TyrRS.
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ポジティブ・コントロール
- HeLa cells and cell lysate and NIH3T3 cell lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
バッファー
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 1% BSA, 0.812% Sodium chloride, 0.0225% Potassium chloride, 0.0204% Dibasic monohydrogen potassium phosphate, 0.1136% Dibasic monohydrogen sodium phosphate, PBS -
Concentration information loading...
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精製度
Protein G purified -
特記事項(精製)
ab50961 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum. -
ポリ/モノ
モノクローナル -
クローン名
YAR5H08 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab50961の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/100. Detects a band of approximately 58 kDa (predicted molecular weight: 58 kDa).
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IP |
Use at 20 µg/mg of lysate.
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ICC |
1/10.
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特記事項 |
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WB
1/100. Detects a band of approximately 58 kDa (predicted molecular weight: 58 kDa). |
IP
Use at 20 µg/mg of lysate. |
ICC
1/10. |
ターゲット情報
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機能
Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr). -
関連疾患
Defects in YARS are the cause of Charcot-Marie-Tooth disease dominant intermediate type C (CMTDIC) [MIM:608323]. CMTDIC is a form of Charcot-Marie-Tooth disease characterized by clinical and pathologic features intermediate between demyelinating and axonal peripheral neuropathies, and motor median nerve conduction velocities ranging from 25 to 45 m/sec. -
配列類似性
Belongs to the class-I aminoacyl-tRNA synthetase family.
Contains 1 tRNA-binding domain. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 8565 Human
- Entrez Gene: 107271 Mouse
- Omim: 603623 Human
- SwissProt: P54577 Human
- SwissProt: Q91WQ3 Mouse
- Unigene: 213264 Human
- Unigene: 145488 Mouse
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別名
- CMTDIC antibody
- SYYC_HUMAN antibody
- Tyrosine tRNA ligase, cytoplasmic antibody
see all
画像
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Anti-Tyrosyl tRNA synthetase/TyrRS antibody [YAR5H08] (ab50961) at 1/100 dilution + HeLa whole cell lysate at 50 µg
Secondary
anti-mouse IgG at 1/2500 dilution
Developed using the ECL technique.
Predicted band size: 58 kDa
Observed band size: 58 kDa -
Immunoprecipititation analysis using 10ug ab50961 per 500ug RIPA HeLa cell lysate. Immunoblot using ab50961 at 1/100 dilution and TrueBlotTM anti-mouse IgG at 1/1000 as secondary antibody.
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Anti-Tyrosyl tRNA synthetase/TyrRS antibody [YAR5H08] (ab50961) at 1/100 dilution + NIH3T3 whole cell lysate at 50 µg
Secondary
anti-mouse IgG at 1/2500 dilution
Developed using the ECL technique.
Predicted band size: 58 kDa
Observed band size: 58 kDa -
ab50961 at 1/10 dilution staining human YARS in HeLa cells by Immunocytochemistry. AlexaFluor 488 Goat anti-mouse IgG at 1/200 as secondary.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (2)
ab50961 は 2 報の論文で使用されています。
- Jhanji M et al. Cis- and trans-resveratrol have opposite effects on histone serine-ADP-ribosylation and tyrosine induced neurodegeneration. Nat Commun 13:3244 (2022). PubMed: 35688816
- Park SJ et al. Evaluation of Multi-tRNA Synthetase Complex by Multiple Reaction Monitoring Mass Spectrometry Coupled with Size Exclusion Chromatography. PLoS One 10:e0142253 (2015). WB ; Human . PubMed: 26544075