Application
Western blot
Sample
Human Cell lysate - whole cell (primary skin keratinicytes)
Gel Running Conditions
Reduced Denaturing (gel any Kd from BioRad)
Loading amount
30 µg
Specification
primary skin keratinicytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Milk TBS-T 0.2%
Dilution
1/1000
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: anti-mouse HRP linked
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL+
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 77 kDa
Additional data
Additional Notes
lane 1 : Primary keratinicytes whole cell extract 30 ug
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MR. Julien Coutier
Verified customer
投稿 May 12 2017