In this experiment we used isolated mitochondria from human HCT116 colon cancer cell line. The proteins from the mitochondrial lysate were seperated on a 13% SDS gel. The total OXPHOS human WB antibody kit was diluted 1:1000 in 2% milk. We already obtained strong signals of the complexes V and I after 10 sec. After 1 min (attached figure) we got signals from all five complexes.
The kit is easy to use and gives reproducibly results.
The kit is easy to use and gives reproducibly results.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MR. Christian Marx
Verified customer
投稿 Aug 21 2013