Anti-TRRAP 抗体 - ChIP Grade (ab73546)
Key features and details
- Rabbit polyclonal to TRRAP - ChIP Grade
- Suitable for: WB, ChIP
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-TRRAP antibody - ChIP Grade
TRRAP 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to TRRAP - ChIP Grade -
由来種
Rabbit -
アプリケーション
適用あり: WB, ChIPmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Xenopus laevis -
免疫原
Synthetic peptide corresponding to Human TRRAP aa 3800 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab101312) -
ポジティブ・コントロール
- This antibody gave a positive signal in the following nuclear lysate preparations: HeLa; Jurkat.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab73546の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (2) |
Use a concentration of 1 µg/ml. Detects a band of approximately 438 kDa (predicted molecular weight: 438 kDa).
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ChIP | (1) |
Use 5 µg for 25 µg of chromatin.
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特記事項 |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 438 kDa (predicted molecular weight: 438 kDa). |
ChIP
Use 5 µg for 25 µg of chromatin. |
ターゲット情報
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関連性
TRRAP (TRansformation/tRanscription domain Associated Protein) is an adapter protein that is found in a number of multiprotein chromatin complexes with histone acetyltransferase (HAT) activity. These complexes include PCAF, TFTC-HAT, TIP60 HAT, STAGA, and BAF53 complexes. TRRAP is thought to be responsible for the concerted and context-dependent recruitment of these complexes to chromatin during transcription, replication and DNA repair. TRRAP plays a key role in transcriptional activation by c-Myc, p53/TP53, E2F1 and E2F4. -
細胞内局在
Nuclear -
参照データベース
- Entrez Gene: 8295 Human
- Entrez Gene: 100683 Mouse
- Entrez Gene: 288471 Rat
- Omim: 603015 Human
- SwissProt: Q9Y4A5 Human
- SwissProt: Q80YV3 Mouse
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別名
- 350/400 kDa PCAF associated factor antibody
- PAF 350/400 antibody
- PAF 400 antibody
see all
画像
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All lanes : Anti-TRRAP antibody - ChIP Grade (ab73546) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
Lane 2 : Jurkat nuclear extract lysate (ab14844)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 438 kDa
Observed band size: 438 kDa
Additional bands at: 150 kDa, 90 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutes -
Chromatin was prepared from HeLa cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 25µg of chromatin, 5µg of ab73546 (blue), and 20µl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Sybr green approach for both the active and heterochromatic loci). Primers are located in the first kb of the transcribed region.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab73546 は 5 報の論文で使用されています。
- Tavares M et al. JAZF1-SUZ12 dysregulates PRC2 function and gene expression during cell differentiation. Cell Rep 39:110889 (2022). PubMed: 35649353
- Sheng X et al. IRE1a-XBP1s pathway promotes prostate cancer by activating c-MYC signaling. Nat Commun 10:323 (2019). PubMed: 30679434
- Wang Z et al. TRRAP is a central regulator of human multiciliated cell formation. J Cell Biol 217:1941-1955 (2018). PubMed: 29588376
- Han SI et al. Estrogen receptor ligands ameliorate fatty liver through a nonclassical estrogen receptor/Liver X receptor pathway in mice. Hepatology 59:1791-802 (2014). PubMed: 24277692
- O'Neill DJ et al. SETD6 controls the expression of estrogen-responsive genes and proliferation of breast carcinoma cells. Epigenetics 9:942-50 (2014). PubMed: 24751716