Application
Western blot
Sample
Rat Cell lysate - whole cell (9L, PC12, RIE-1)
Loading amount
20 µg
Specification
9L, PC12, RIE-1
Gel Running Conditions
Reduced Denaturing
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
Other product details
Concentration
1 µg/ml
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 3% BSA, PBS-0.1% Tween 20
Secondary antibody
Name
Non-Abcam antibody was used: Anti-rabbit
Host species: Pig
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Pig
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
Supersignal West Dura
Exposure
3 minute(s) and 0 second(s)
Bands
Specific: 125 kDa
Additional data
Additional Notes
Lane order: (1) 9L (2) PC12 (3) RIE-1
This antibody detects a band of ~125 kDa in 3 different rat cell lines which is lower than the predicted molecular weight of TRPV4 on SDS-PAGE (100 kDa). However, there are published reports of TRPV4 running at >120 kDa PMID: 16368742 PMID: 12538589, possibly as a result of glycosylation.
This antibody detects a band of ~125 kDa in 3 different rat cell lines which is lower than the predicted molecular weight of TRPV4 on SDS-PAGE (100 kDa). However, there are published reports of TRPV4 running at >120 kDa PMID: 16368742 PMID: 12538589, possibly as a result of glycosylation.
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投稿 Jun 22 2010