Anti-TRIP12/ULF 抗体 (ab86220)
Key features and details
- Rabbit polyclonal to TRIP12/ULF
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
製品の概要
-
製品名
Anti-TRIP12/ULF antibody
TRIP12/ULF 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to TRIP12/ULF -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Human
交差が予測される動物種: Guinea pig, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Camel, Elephant -
免疫原
Synthetic peptide corresponding to Human TRIP12/ULF.
Database link: NP_004229.1 -
ポジティブ・コントロール
- HeLa whole cell lysate (ab150035)
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
-
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab86220の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB |
1/2000 - 1/10000. Predicted molecular weight: 220 kDa.
|
|
IP |
Use at 2-5 µg/mg of lysate.
|
特記事項 |
---|
WB
1/2000 - 1/10000. Predicted molecular weight: 220 kDa. |
IP
Use at 2-5 µg/mg of lysate. |
ターゲット情報
-
関連性
TRIP12 (thyroid hormone receptor interactor 12) is an ATP-dependent multisubunit protein that activates the proteolytic activities of the multifunctional proteinase (20S proteasome) of the 26S complex. TRP12 specifically interacts with the ligand binding domain of the thyroid hormone receptor (in a thyroid hormone T3-independent manner) and with retinoid X receptor (RXR). TRP12 was originally identified as a factor that interacts with the thyroid hormone receptor. It was later found to also interact with AAP-BP1, a component of the NEDD8 –activating enzyme, and function as an E3 ubiquitin ligase for AAP-BP1. -
参照データベース
- Entrez Gene: 9320 Human
- Omim: 604506 Human
- SwissProt: Q14669 Human
-
別名
- KIAA0045 antibody
- MGC138849 antibody
- MGC138850 antibody
see all
画像
-
All lanes : Anti-TRIP12/ULF antibody (ab86220) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 5 µg
Lane 2 : HeLa whole cell lysate at 1.5 µg
Lane 3 : HeLa whole cell lysate at 0.5 µg
Developed using the ECL technique.
Predicted band size: 220 kDa
Exposure time: 3 minutes -
Detection of human TRIP12/ULF in Immunoprecipitates of HeLa whole cell lysate (1 mg lysate for IP, 20% of IP loaded) using ab86220 at 3 µg/mg lysate for IP, and at 0.1 µg/ml for subsequent Western blot.
Detection: Chemiluminescence with an exposure time of 10 seconds.
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (5)
ab86220 は 5 報の論文で使用されています。
- Molkentine DP et al. p16 Represses DNA Damage Repair via a Novel Ubiquitin-Dependent Signaling Cascade. Cancer Res 82:916-928 (2022). PubMed: 34965932
- Harris LD et al. The deubiquitinase TRABID stabilizes the K29/K48-specific E3 ubiquitin ligase HECTD1. J Biol Chem 296:100246 (2021). PubMed: 33853758
- Cipolla L et al. UBR5 interacts with the replication fork and protects DNA replication from DNA polymerase ? toxicity. Nucleic Acids Res 47:11268-11283 (2019). PubMed: 31586398
- Flack JE et al. Wnt-Dependent Inactivation of the Groucho/TLE Co-repressor by the HECT E3 Ubiquitin Ligase Hyd/UBR5. Mol Cell 67:181-193.e5 (2017). PubMed: 28689657
- Mounir Z et al. ERG signaling in prostate cancer is driven through PRMT5-dependent methylation of the Androgen Receptor. Elife 5:N/A (2016). WB . PubMed: 27183006