製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to TRAM1
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: IHC-P, ICC/IF, WBmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Synthetic peptide within Human TRAM1 aa 310-374. The exact sequence is proprietary.
    Sequence:

    FMMWKFINFQLRRWREHSAFQAPAVKKKPTVTKGRSSKKGT ENGVNGTLTSNVADSPRNKKEKSS


    Database link: NP_055109

  • ポジティブ・コントロール
    • WB: H1299 whole cell lysate. IHC: mouse intestinal and AGS xenograft tissue. ICC/IF: HeLa cells.

法規制情報

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab96106 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/1000.
WB 1/500 - 1/3000. Predicted molecular weight: 43 kDa.

ターゲット情報

  • 関連性
    Function: Stimulatory or required for the translocation of secretory proteins across the ER membrane. Similarity: Belongs to the TRAM family. Contains 1 TLC (TRAM/LAG1/CLN8) domain.
  • 細胞内局在
    Endoplasmic reticulum membrane; Multipass membrane protein
  • 参照データベース
  • 別名
    • PNAS 8 antibody
    • PNAS8 antibody
    • PRO1292 antibody
    • TRAM 1 antibody
    • TRAM antibody
    • TRAM1 antibody
    • TRAM1_HUMAN antibody
    • TRAMP antibody
    • Translocating chain associated membrane protein 1 antibody
    • Translocating chain associating membrane protein antibody
    • Translocating chain-associated membrane protein 1 antibody
    • Translocation associated membrane protein 1 antibody
    • Translocation associating membrane protein 1 antibody
    see all

画像

  • Anti-TRAM1 antibody (ab96106) at 1/1000 dilution + H1299 cell lysate at 30 µg

    Predicted band size: 43 kDa



    10% SDS-PAGE
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of mouse intestine staining TRAM1 with ab96106 at 1/500 dilution. 

  • Immunocytochemistry/ Immunofluorescence of methanol-fixed HeLa cells staining TRAM1 with ab96106 at 1/500 dilution (green). Costained with Hoechst 33342 (blue).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of AGS xenograft staining TRAM1 with ab96106 at 1/500 dilution. 

プロトコール

参考文献

This product has been referenced in:
  • Iampietro M  et al. Ebola virus glycoprotein directly triggers T lymphocyte death despite of the lack of infection. PLoS Pathog 13:e1006397 (2017). Read more (PubMed: 28542576) »
  • Yew KH  et al. Human cytomegalovirus induces TLR4 signaling components in monocytes altering TIRAP, TRAM and downstream interferon-beta and TNF-alpha expression. PLoS One 7:e44500 (2012). WB . Read more (PubMed: 22970235) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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