Anti-Tissue Plasminogen Activator 抗体 (ab62763)
Key features and details
- Rabbit polyclonal to Tissue Plasminogen Activator
- Suitable for: ELISA, WB
- Reacts with: Mouse, Rat
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
-
製品名
Anti-Tissue Plasminogen Activator antibody
Tissue Plasminogen Activator 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Tissue Plasminogen Activator -
由来種
Rabbit -
アプリケーション
適用あり: ELISA, WBmore details -
種交差性
交差種: Mouse, Rat -
免疫原
Recombinant full length protein corresponding to Mouse TPA Tissue Plasminogen Activator.
-
ポジティブ・コントロール
- WB: Mouse TPA Tissue Plasminogen Activator (non-reduced).
-
特記事項
The concentration of this antibody is lot specific.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle. -
バッファー
Constituents: 0.82% Sodium phosphate, 0.0292% EDTA, 0.58% Sodium chloride -
Concentration information loading...
-
精製度
Protein A purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Assay kits
-
Compatible Secondaries
-
Conjugation kits
-
Isotype control
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab62763の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
ELISA |
Use at an assay dependent concentration.
|
|
WB |
Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 62 kDa).
|
特記事項 |
---|
ELISA
Use at an assay dependent concentration. |
WB
Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 62 kDa). |
ターゲット情報
-
機能
Converts the abundant, but inactive, zymogen plasminogen to plasmin by hydrolyzing a single Arg-Val bond in plasminogen. By controlling plasmin-mediated proteolysis, it plays an important role in tissue remodeling and degradation, in cell migration and many other physiopathological events. Play a direct role in facilitating neuronal migration. -
組織特異性
Synthesized in numerous tissues (including tumors) and secreted into most extracellular body fluids, such as plasma, uterine fluid, saliva, gingival crevicular fluid, tears, seminal fluid, and milk. -
関連疾患
Note=Increased activity of TPA results in increased fibrinolysis of fibrin blood clots that is associated with excessive bleeding. Defective release of TPA results in hypofibrinolysis that can lead to thrombosis or embolism. -
配列類似性
Belongs to the peptidase S1 family.
Contains 1 EGF-like domain.
Contains 1 fibronectin type-I domain.
Contains 2 kringle domains.
Contains 1 peptidase S1 domain. -
ドメイン
Both FN1 and one of the kringle domains are required for binding to fibrin.
Both FN1 and EGF-like domains are important for binding to LRP1.
The FN1 domain mediates binding to annexin A2.
The second kringle domain is implicated in binding to cytokeratin-8 and to the endothelial cell surface binding site. -
翻訳後修飾
The single chain, almost fully active enzyme, can be further processed into a two-chain fully active form by a cleavage after Arg-310 catalyzed by plasmin, tissue kallikrein or factor Xa.
Differential cell-specific N-linked glycosylation gives rise to two glycoforms, type I (glycosylated at Asn-219) and type II (not glycosylated at Asn-219). The single chain type I glycoform is less readily converted into the two-chain form by plasmin, and the two-chain type I glycoform has a lower activity than the two-chain type II glycoform in the presence of fibrin.
N-glycosylation of Asn-152; the bound oligomannosidic glycan is involved in the interaction with the mannose receptor.
Characterization of O-linked glycan was studied in Bowes melanoma cell line. -
細胞内局在
Secreted > extracellular space. - Information by UniProt
-
参照データベース
- Entrez Gene: 18791 Mouse
- Entrez Gene: 25692 Rat
- SwissProt: P11214 Mouse
- SwissProt: P19637 Rat
- Unigene: 154660 Mouse
- Unigene: 107102 Rat
-
別名
- Alteplase antibody
- DKFZp686I03148 antibody
- Plasminogen activator tissue antibody
see all
画像
-
All lanes : Anti-Tissue Plasminogen Activator antibody (ab62763) at 2 µg/ml
Lane 1 : Mouse TPA Tissue Plasminogen Activator (non-reduced) at 0.5 µg
Lane 2 : Mouse TPA Tissue Plasminogen Activator + 3 µg CPAI (non-reduced) at 0.5 µg
Lane 3 : Mouse TPA Tissue Plasminogen Activator (reduced) at 0.5 µg
Lane 4 : Mouse TPA Tissue Plasminogen Activator + 3ug CPAI (reduced) at 0.5 µg
Lane 5 : Marker
Secondary
All lanes : Anti Rabbit IgG ABC Kit
Predicted band size: 62 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?
Additional bands at: 100 kDa, 125 kDa, 38 kDa, 85 kDa. We are unsure as to the identity of these extra bands.
データシートおよび資料
-
Datasheet download
参考文献 (3)
ab62763 は 3 報の論文で使用されています。
- Lenoir S et al. Post-synaptic Release of the Neuronal Tissue-Type Plasminogen Activator (tPA). Front Cell Neurosci 13:164 (2019). PubMed: 31105531
- Bonis A et al. Cardiovascular resistance to thrombosis in 13-lined ground squirrels. J Comp Physiol B 189:167-177 (2019). PubMed: 30317383
- Sagare AP et al. A lipoprotein receptor cluster IV mutant preferentially binds amyloid-ß and regulates its clearance from the mouse brain. J Biol Chem 288:15154-66 (2013). PubMed: 23580652