Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Kidney)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Sodium citrate buffer
Permeabilization
No
Specification
Kidney
Blocking step
Fish skin gelatin as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.2% · Temperature: 21°C
Fixative
Paraformaldehyde
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 0.2% fish skin gelatin in PBS
Dilution
1/200
Secondary antibody
Dilution
1/200
Name
Non-Abcam antibody was used: Alexa Fluor 568 goat anti-mouse IgG (H+L)
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 568
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 568
Additional data
Abcam response
This product has not been tested with mouse samples in IHC-P however as the immunogen show high homology, so the products should work. The end user has tested it under our Abtrial promotion and they are now eligible to receive a free product in exchange of this review. Please contact Abcam Scientific Support team for more details on troubleshooting.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
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投稿 Sep 06 2018