Application
Western blot
Sample
Human Cell lysate - whole cell (Placenta)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
Placenta
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
14 hour(s) and 0 minute(s) · Diluent: TBST(BSA 5%)
Dilution
1/1000
Detection
Detection method
ECL
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 25 kDa Non-specific: 55, 35 kDa
Additional data
Additional Notes
Placental tissue were homogenized with RIPA buffer (abcam protocol) with Roche cOmplete mini (EDTA free and Roche PhoSTOP inhibitor cocktail was used.
All lanes are loaded with Placental tissue lysates from control and different diseased patient.
0.45um PVDF (PALL) were used.
ECL: Biorad Clarity western ECL kit.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Dec 22 2016