Application
Flow Cytometry
Sample
Human Cell (Platelets)
Specification
Platelets
Preparation
Cell harvesting/tissue preparation method: PL were isolated spinning Platelet rich plasma on Histopaque
Sample buffer: PBS
Sample buffer: PBS
Fixation
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton-X100 in 2% BSA for 30min
Gating Strategy
Platelets
Other product details
Dilution
1/200
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 2% BSA in PBS
Secondary antibody
Name
Non-Abcam antibody was used: Alexa Flour594 Goat Anti Mouse IgG (H+L)
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Dilution
1/500
Additional data
Additional Notes
Abbreviations in the fig
Plts : Platelets;
UP : Unpermeabilized;
P : Permeabilized;
US : Unstained, Red Peak;
IgG Ms : IgG Mouse (Isotype Control), Blue Peak;
TLR4 : Green peak.
Plts : Platelets;
UP : Unpermeabilized;
P : Permeabilized;
US : Unstained, Red Peak;
IgG Ms : IgG Mouse (Isotype Control), Blue Peak;
TLR4 : Green peak.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Mahesh Shivananjappa
Verified customer
投稿 Dec 16 2010