Anti-TIL/TLR1 抗体 [EPR2075] (ab68158)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2075] to TIL/TLR1
- Suitable for: WB, IP, Flow Cyt
- Reacts with: Human
Related conjugates and formulations
製品の概要
-
製品名
Anti-TIL/TLR1 antibody [EPR2075]
TIL/TLR1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR2075] to TIL/TLR1 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IP, Flow Cytmore details
適用なし: ICC/IF or IHC-P -
種交差性
交差種: Human -
免疫原
Synthetic peptide within Human TIL/TLR1 aa 150-250 (extracellular). The exact sequence is proprietary.
-
ポジティブ・コントロール
- WB: Daudi and Ramos cell lysates. Flow Cyt: Daudi cells. IP: Ramos cell lysate.
-
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
-
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR2075 -
アイソタイプ
IgG -
研究分野
関連製品
-
Alternative Versions
-
Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab68158の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB | ||
IP | ||
Flow Cyt |
特記事項 |
---|
IP: 1/40.
WB: 1/1000 - 1/2000. Detects a band of approximately 90 and 125 kDa (predicted molecular weight: 90 kDa).
Is unsuitable for ICC or IHC-P.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
ターゲット情報
-
機能
Participates in the innate immune response to microbial agents. Cooperates with TLR2 to mediate the innate immune response to bacterial lipoproteins or lipopeptides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. -
組織特異性
Ubiquitous. Highly expressed in spleen, ovary, peripheral blood leukocytes, thymus and small intestine. -
配列類似性
Belongs to the Toll-like receptor family.
Contains 12 LRR (leucine-rich) repeats.
Contains 1 LRRCT domain.
Contains 1 TIR domain. -
細胞内局在
Cell membrane. Cytoplasmic vesicle > phagosome membrane. - Information by UniProt
-
参照データベース
- Entrez Gene: 7096 Human
- Omim: 601194 Human
- SwissProt: Q15399 Human
- Unigene: 621817 Human
- Unigene: 654532 Human
-
別名
- CD281 antibody
- CD281 antigen antibody
- KIAA0012 antibody
see all
画像
-
All lanes : Anti-TIL/TLR1 antibody [EPR2075] (ab68158) at 1/1000 dilution
Lane 1 : Daudi cell lysate
Lane 2 : Ramos cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 90 kDa
Observed band size: 90 kDa
Additional bands at: 125 kDa. We are unsure as to the identity of these extra bands. -
Flow cytometric analysis of TIL/TLR1 in Daudi cells using ab68158 at 1/60 dilution (pink) or a rabbit IgG (green).
-
TIL/TLR1 was immunoprecipitated from 0.35 mg Ramos (Human Burkitt's lymphoma B lymphocyte) whole cell lysate 10 µg with ab68158 at 1/50 dilution (2µg). VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: Ramos (Human Burkitt's lymphoma B lymphocyte) whole cell lysate 10 µg
Lane 2: ab68158 IP in Ramos whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab68158 in Ramos whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (1)
ab68158 は 1 報の論文で使用されています。
- Galoian K et al. Toll like receptors TLR1/2, TLR6 and MUC5B as binding interaction partners with cytostatic proline rich polypeptide 1 in human chondrosarcoma. Int J Oncol 52:139-154 (2018). PubMed: 29138803