製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to TIS11B
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: ELISA, WB, EMSAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Cow, Dog
  • 免疫原

    Synthetic peptide corresponding to Human TIS11B aa 46-95 (N terminal). The immunogen was taken from within these 50aa's
    Sequence:

    GGGFPRRHSVTLPSSKFHQNQLLSSLKGEPAPALSSRDSRFRDRSFSEGG


    Database link: Q07352

  • ポジティブ・コントロール
    • Human placenta lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab42473 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/62500.
WB Use a concentration of 0.125 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 36 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
EMSA Use at an assay dependent dilution. PubMed: 20691152

ターゲット情報

画像

  • Anti-TIS11B antibody (ab42473) at 0.125 µg/ml (5% skimmed milk / PBS) + 10µg Human placenta whole cell lysate

    Secondary
    HRP conjugated anti-rabbit at 1/50000 dilution

    Predicted band size: 36 kDa
    Observed band size: 30 kDa
    why is the actual band size different from the predicted?



    Gel concentration 12%.

プロトコール

参考文献

This product has been referenced in:
  • Salerno F  et al. Translational repression of pre-formed cytokine-encoding mRNA prevents chronic activation of memory T cells. Nat Immunol N/A:N/A (2018). Read more (PubMed: 29988089) »
  • Kim CW  et al. Ectopic over-expression of tristetraprolin in human cancer cells promotes biogenesis of let-7 by down-regulation of Lin28. Nucleic Acids Res : (2011). WB ; Human . Read more (PubMed: 22210895) »
See all 4 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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