保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
バッファーPreservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS
Concentration information loading...
精製度Immunogen affinity purified
Our Abpromise guarantee covers the use of ab42473 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.125 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 36 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
|EMSA||Use at an assay dependent dilution. PubMed: 20691152|
機能Probable regulatory protein involved in regulating the response to growth factors.
配列類似性Contains 2 C3H1-type zinc fingers.
細胞内局在Cytoplasm. Nucleus. Shuttles between the nucleus and the cytoplasm.
- Information by UniProt
- Berg36 antibody
- BRF1 antibody
- Butyrate response factor 1 antibody
Anti-TIS11B antibody (ab42473) at 0.125 µg/ml (5% skimmed milk / PBS) + 10µg Human placenta whole cell lysate
HRP conjugated anti-rabbit at 1/50000 dilution
Predicted band size: 36 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted?
Gel concentration 12%.
This product has been referenced in:
- Salerno F et al. Translational repression of pre-formed cytokine-encoding mRNA prevents chronic activation of memory T cells. Nat Immunol N/A:N/A (2018). Read more (PubMed: 29988089) »
- Kim CW et al. Ectopic over-expression of tristetraprolin in human cancer cells promotes biogenesis of let-7 by down-regulation of Lin28. Nucleic Acids Res : (2011). WB ; Human . Read more (PubMed: 22210895) »