TIP30 一次抗体 製品一覧
製品の詳細Rabbit polyclonal to TIP30
アプリケーション適用あり: WBmore details
交差が予測される動物種: Mouse, Rat, Cow, Non human primates
- Recombinant Human TIP30 protein (ab117665) can be used as a positive control in WB. This antibody gave a positive signal in SW480 Whole Cell Lysate
保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
バッファーPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
精製度Immunogen affinity purified
Our Abpromise guarantee covers the use of ab71752 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 29 kDa (predicted molecular weight: 27 kDa).|
機能Oxidoreductase required for tumor suppression. NAPDH-bound form inhibits nuclear import by competing with nuclear import substrates for binding to a subset of nuclear transport receptors. May act as a redox sensor linked to transcription through regulation of nuclear import. Isoform 1 is a metastasis suppressor with proapoptotic as well as antiangiogenic properties. Isoform 2 has an antiapoptotic effect.
組織特異性Ubiquitous. Highest level in liver. High levels in lung, skeletal muscle, pancreas and placenta. Moderate levels in heart and kidney. Low levels in brain. Not expressed or low levels in variant small cell lung carcinomas, 33% of hepatocellular carcinomas and neuroblastomas.
ドメインUnique C-terminus confers high proteasome-dependent instability to isoform 2.
細胞内局在Cytoplasm. Nucleus envelope.
- Information by UniProt
- 30 kDa HIV 1 TAT interacting protein antibody
- 30 kDa HIV-1 TAT-interacting protein antibody
- 30 kDa HIV1 TAT interacting protein antibody
Anti-TIP30 antibody (ab71752) at 1 µg/ml + SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate at 10 µg
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 27 kDa
Observed band size: 29 kDa why is the actual band size different from the predicted?
Additional bands at: 37 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 1 minute
TIP30 contains a number of potential phosphorylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
ab71752 has not yet been referenced specifically in any publications.