Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Renal proximal tubular epithelial cells)
Specification
Renal proximal tubular epithelial cells
Fixative
Paraformaldehyde
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 21°C
Other product details
Dilution
1/100
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 21°C · Diluent: 0.1 % BSA in PBS
Secondary antibody
Name
Non-Abcam antibody was used: Invitrogen
Host species: Goat
Clonality: Monoclonal
Conjugation: Alexa Fluor® 555
Host species: Goat
Clonality: Monoclonal
Conjugation: Alexa Fluor® 555
Dilution
1/1000
Additional data
Abcam response
This antibody was used in PubMed ID 16775137 in mature rat primary hippocampal neurons in ICC/IF. The authors used a primary dilution of 1/20. Perhaps poor staining was seen because the primary concentration was too low.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Robert Jenkins
Verified customer
投稿 Apr 13 2009