Anti-TGF alpha 抗体 (ab112030)
Key features and details
- Rabbit polyclonal to TGF alpha
- Suitable for: IHC-P
- Reacts with: Rat, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-TGF alpha antibody
TGF alpha 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to TGF alpha -
由来種
Rabbit -
アプリケーション
適用あり: IHC-Pmore details -
種交差性
交差種: Rat, Human
交差が予測される動物種: Mouse, Rabbit, Cow, Chimpanzee, Macaque monkey, Gorilla, Orangutan -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- This antibody gave a positive result in IHC in the following FFPE tissues: Human skin melanoma and Rat normal kidney.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab112030の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
Use a concentration of 1 - 5 µg/ml.
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特記事項 |
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IHC-P
Use a concentration of 1 - 5 µg/ml. |
ターゲット情報
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機能
TGF alpha is a mitogenic polypeptide that is able to bind to the EGF receptor and to act synergistically with TGF beta to promote anchorage-independent cell proliferation in soft agar. -
組織特異性
Isoform 1, isoform 3 and isoform 4 are expressed in keratinocytes and tumor-derived cell lines. -
配列類似性
Contains 1 EGF-like domain. -
細胞内局在
Cell membrane and Secreted > extracellular space. - Information by UniProt
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参照データベース
- Entrez Gene: 540388 Cow
- Entrez Gene: 7039 Human
- Entrez Gene: 21802 Mouse
- Entrez Gene: 100009482 Rabbit
- Entrez Gene: 24827 Rat
- Omim: 190170 Human
- SwissProt: P01135 Human
- SwissProt: P48030 Mouse
see all -
別名
- EGF like TGF antibody
- EGF-like TGF antibody
- ETGF antibody
see all
画像
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IHC image of TGF alpha staining in Rat normal kidney formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab112030, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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IHC image of TGF alpha staining in Human skin melanoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab112030, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab112030 は 3 報の論文で使用されています。
- Mao Y et al. MELTF Might Regulate Ferroptosis, Pyroptosis, and Autophagy in Platelet-Rich Plasma-Mediated Endometrial Epithelium Regeneration. Reprod Sci N/A:N/A (2022). PubMed: 36303086
- Rothhammer V et al. Microglial control of astrocytes in response to microbial metabolites. Nature 557:724-728 (2018). PubMed: 29769726
- Hollmén M et al. G-CSF regulates macrophage phenotype and associates with poor overall survival in human triple-negative breast cancer. Oncoimmunology 5:e1115177 (2016). IHC-P ; Human . PubMed: 27141367