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Synthetic peptide within Mouse TBR1 aa 50-150 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
(Peptide available as
Our Abpromise guarantee covers the use of ab31940 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use at an assay dependent concentration.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 74 kDa (predicted molecular weight: 74 kDa).Can be blocked with Mouse TBR1 peptide (ab25853).|
|IHC-FoFr||Use at an assay dependent concentration. PubMed: 19709629|
|IHC-P||1/25. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.|
Human iPSCs (induced pluripotent stem cell) were fixed with 4% paraformaldehyde, followed by membrane permeabilization and blocking with 0.1% Triton X-100 in donkey serum. Samples were incubated with ab31940 at a 1:200 dilution overnight then with the secondary antibody for 1 hour. Imaging was performed using a Zeiss LSM710 confocal microscope and images were acquired using ZEN black software. Software was used to pseudo-color images and add scale bars.
Quantified MAP2 immunostaining was performed blind on at least 3 images per condition, with at least 200 cells counted per image, using ImageJ software (NIH).
IHC-P image of TBR1 staining on mouse brain sections using ab31940 at a 1:200 dilution.
The sections were deparaffinized and subjected to heat mediated antigen retrieval. The sections were blocked using 7.5% goat serum for 2 hours at room temperature. ab31940 was diluted 1:200 using blocking buffer and incubated with the sections for 16 hours at 4°C. The secondary antibody used was Goat polyclonal to anti-rabbit conjugated to Alexa Fluor® 594 (1:400).
DAPI was used to counterstain nuclei.
ab31940 staining mouse brain tissue by IHC-Fr.
The sections were fixed with PFA and permeabilized in PBST. The primary antibody was diluted 1/500 and incubated with the sample for 14 hours. An Alexa Fluor® 594 goat anti-rabbit antibody was used as the secondary.
ab31940 staining rat brain sections by IHC-Fr.
The animal was perfused with 4% paraformaldehyde and further post fixed with 4% paraformaldehyde overnight. The tissues were cryoprotected with 30% sucrose and sectioned using a cryostat. The tissue was blocked with 10% donkey serum in 0.3% TritonX in 0.1% PBS for 30 minutes at 24°C. Staining with ab31940 at a 1/200 dilution in 0.3% TritonX with 0.1x PBS- 10% donkey serum was performed for 4 hours at 24°C. A donkey anti-rabbit Alexa-Fluor® 488 polyclonal antibody at 1/1000 was used as the secondary antibody.
TBR1 expressed in the several cortical layers distinctly in pyramidal neurons.
(Image courtesy of Human Protein Atlas)
ab31940 staining TBR1 protein in normal human cerebral cortex. Brown color indicates presence of protein, blue color shows cell nuclei. Paraffin embedded human cerebral cortex tissue was incubated with ab31940 at a 1/25 dilution for 30 minutes at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.
ab31940 staining rat cortical neurons by ICC.
The cultured cells were fixed with PFA and permeabilized in 0.1% Triton X prior to blocking in 10% BSA for 1 hour at 22°C. The primary antibody was diluted 1/500 and incubated with the sample for 2 hours at 22°C. A Cy2® donkey anti-rabbit antibody was used as the secondary
ab31940 staining TBR1 in human ES cells by Flow Cytometry. Cells were fixed with formaldehyde and permeabilized with Triton X-100. The sample was incubated with the primary antibody (1/200) for 30 minutes. An Alexa Fluor® 647-conjugated goat anti-rabbit IgG (1/20000) was used as the secondary antibody. Gating Strategy: All cells.
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