Anti-TBC1D15 抗体 (ab121396)
Key features and details
- Rabbit polyclonal to TBC1D15
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-TBC1D15 antibody
TBC1D15 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to TBC1D15 -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, IHC-P, WBmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
antigen sequence, corresponding to internal sequence amino acids 169-286 of Human TBC1D15, isoform 2.
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ポジティブ・コントロール
- IHC-P: Human placenta, skin, kidney, and testis tissue sections. WB: NIH/3T3, NBT-II, and NTERA-2 cell lysates. ICC/IF: U-2 OS cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab121396の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
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IHC-P |
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB | (1) |
Use a concentration of 0.04 - 0.4 µg/ml.
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特記事項 |
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ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
IHC-P
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
Use a concentration of 0.04 - 0.4 µg/ml. |
ターゲット情報
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機能
Acts as a GTPase activating protein for RAB7A. Does not act on RAB4, RAB5 or RAB6. -
配列類似性
Contains 1 Rab-GAP TBC domain. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 64786 Human
- Entrez Gene: 66687 Mouse
- Entrez Gene: 366896 Rat
- Omim: 612662 Human
- SwissProt: Q8TC07 Human
- SwissProt: Q9CXF4 Mouse
- Unigene: 284630 Human
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別名
- DKFZp686M1379 antibody
- DKFZp761D0223 antibody
- FLJ12085 antibody
see all
画像
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Immunohistochemical analysis of paraffin embedded human kidney tissue labeling TBC1D15 with ab121396 antibody at a 1/20 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunofluorescent staining of Human cell line U-2 OS shows positivity in cytoplasm and mitochondria. Recommended concentration of ab121396 1-4 µg/ml. Cells treated with PFA/Triton X-100.
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All lanes : Anti-TBC1D15 antibody (ab121396) at 0.4 µg/ml
Lane 1 : NIH/3T3 (mouse embryo fibroblast cell line) cell lysate
Lane 2 : NBT-II (rat urinary bladder tumor cell line) cell lysate -
Immunohistochemical analysis of paraffin embedded human testis tissue labeling TBC1D15 with ab121396 antibody at a 1/20 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin embedded human placenta tissue labeling TBC1D15 with ab121396 antibody at a 1/20 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin embedded human skin tissue labeling TBC1D15 with ab121396 antibody at a 1/20 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Anti-TBC1D15 antibody (ab121396) at 0.4 µg/ml + NTERA-2 (human malignant pluripotent embryonic carcinoma cell line) cell lysate
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab121396 は 3 報の論文で使用されています。
- Jongsma ML et al. SKIP-HOPS recruits TBC1D15 for a Rab7-to-Arl8b identity switch to control late endosome transport. EMBO J 39:e102301 (2020). PubMed: 32080880
- Wu J et al. TBC1D15 affects glucose uptake by regulating GLUT4 translocation. Gene 683:210-215 (2019). PubMed: 30316925
- Xian H et al. STX17 dynamically regulated by Fis1 induces mitophagy via hierarchical macroautophagic mechanism. Nat Commun 10:2059 (2019). PubMed: 31053718