製品の概要

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C.
  • バッファー
    Preservative: 0.02% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    Affinity chromatography purified via peptide column
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab76950 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 24 kDa.

ターゲット情報

  • 関連性
    Streptavidin is a tetrameric protein purified from Streptomyces avidinii. It has wide use in numerous molecular biological protocols dues to its strong affinity for biotin. The original Strep-tag (AWRHPQFGG) was a nine amino acid peptide with high specificity and affinity towards streptavidin which allows the simple purification of protein by use of affinity columns, but required addition to only the C-terminus of recombinant proteins. To also allow a Strep-tag to be placed at the N-terminus of recombinant proteins, it was re-engineered and re-named Strep-tag II.
  • 別名
    • anti-Strep-Tag II antibody
    • Strep-Tag II antibody
    • Strep-Tag II antibody antibody
    • Strep-Tag IIantiboy antibody

画像

  • All lanes : Anti-Strep-tag II antibody (ab76950) at 1 µg/ml

    Lane 1 : Streptavidin tagged recombinant protein with blocking peptide
    Lane 2 : Streptavidin tagged recombinant protein

    Lysates/proteins at 0.05 µg per lane.

    Predicted band size: 24 kDa
    Observed band size: 18 kDa
    why is the actual band size different from the predicted?

プロトコール

参考文献

This product has been referenced in:
  • Baggett NE  et al. Global analysis of translation termination in E. coli. PLoS Genet 13:e1006676 (2017). Read more (PubMed: 28301469) »
  • Lin Y  et al. Antiaging Gene Klotho Deficiency Promoted High-Fat Diet-Induced Arterial Stiffening via Inactivation of AMP-Activated Protein Kinase. Hypertension 67:564-73 (2016). Read more (PubMed: 26781278) »
See all 2 Publications for this product

レビューと Q&A

Answer

We have two different versions of the tag: ab76950- against GGGWSHPQFEK ab76949- against ASWSHPQFEKGA Both contain the tag sequence WSHPQFEK along with extra inert amino acids to make the sequence longer for conjugation chemistry to the carrier protein and purification column. They should not interfere with antibody specificity, but if the customer is concerned, the immunogen for ab76950 would highlight the tag the most.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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