Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Sheep Tissue sections (Bone)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: pH6, Citrate based
Permeabilization
No
Specification
Bone
Blocking step
Serum Free Protein Block (Dako) as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: 24°C
Fixative
10% NBF
Other product details
Incubation time
30 minute(s) · Temperature: 24°C
Dilution
1/800
Secondary antibody
Name
Non-Abcam antibody was used: Envision+ Rabbit (Dako)
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Premier Laboratory
Verified customer
投稿 Apr 05 2016