Application
Flow Cytometry
Sample
African Green Monkey Cell (Simian virus 40 (SV40))
Specification
Simian virus 40 (SV40)
Fixation
Methanol
Permeabilization
Yes - Methanol
Other product details
Dilution
1/100
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: 5% BSA in PBS
Secondary antibody
Name
Abcam antibody:Donkey anti-Mouse IgG H&L (DyLight® 488) secondary antibody
Dilution
1/200
Additional data
Additional Notes
CV1 cells were either mock infected or infected with SV40 at moi 10. Cells were harvest and fixed 72 hrs post infection in 100% Methanol at -20C. Cells were stained for 1 hr with 1:100 dilution of anti-T-antigen, washed and stained with DyLight488-conjugated anti-mouse 1:200.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Mr. Nir Drayman
Verified customer
投稿 Aug 10 2011