Application
Western blot
Loading amount
40 µg
Gel Running Conditions
Reduced Denaturing (12)
Sample
Human Cell lysate - whole cell (COV434 cell line)
Specification
COV434 cell line
Blocking step
Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 26°C
Other product details
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 26°C · Diluent: Odyssey Blocking Buffer + 0.2% Tween
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: IRDye 800CW Goat anti-Rabbit IgG (H + L)
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye 800CW
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye 800CW
Dilution
1/10000
Detection
Detection method
Infrared
Bands
Specific: 47 kDa Non-specific: 62 kDa
Exposure
4 minute(s) and 0 second(s)
Additional data
Additional Notes
Picture shows two replicates of COV434 cell line.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Feb 13 2015