製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to SOX4
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: IHC-P, ICC/IF, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Goat, Horse, Chicken, Guinea pig, Cow, Dog, Drosophila melanogaster, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to Human SOX4 (N terminal). Immunogen located within the region from aa 38 and 87.
    Database link: Q06945

  • ポジティブ・コントロール
    • WB: HeLa whole cell lysate (ab150035). ICC/IF: SKNSH cells. IHC-P: Human testis tissue.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab80261 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 5 µg/ml.
ICC/IF Use a concentration of 1 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

画像

  • Anti-SOX4 antibody (ab80261) at 1 µg/ml + HeLa cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size: 47 kDa
    Observed band size: 47 kDa
    Additional bands at: 75 kDa. We are unsure as to the identity of these extra bands.



    Gel concentration 12%.
  • ab80261 at 5 µg/ml staining SOX4 in human testis tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

  • ICC/IF image of ab80261 stained SKNSH cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab80261, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

参考文献

This product has been referenced in:
  • Yang YN  et al. miR-204 reverses temozolomide resistance and inhibits cancer initiating cells phenotypes by degrading FAP-a in glioblastoma. Oncol Lett 15:7563-7570 (2018). Read more (PubMed: 29725461) »
  • Liu Q  et al. UCA1 promotes cell proliferation and invasion and inhibits apoptosis through regulation of the miR129-SOX4 pathway in renal cell carcinoma. Onco Targets Ther 11:2475-2487 (2018). Read more (PubMed: 29760557) »
See all 7 Publications for this product

レビューと Q&A

1-3 of 3 Abreviews or Q&A

Answer

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement of ab85204.

To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

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Question
Answer

Merci pour votre appel.

Voici les alignements promise pour les deux anticorps:

ab90696

http://www.ebi.ac.uk/Tools/services/web_clustalw2/toolresult.ebi?tool=clustalw2&jobId=clustalw2-I20120417-164650-0141-14077005-oy

ab80261

http://www.ebi.ac.uk/Tools/services/web_clustalw2/toolresult.ebi?tool=clustalw2&jobId=clustalw2-I20120417-170647-0683-30096995-oy

Je viens aussi de vous avoir envoyé le devis.

N'hésitez pas à nous rappeler pour des autres renseignements.

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Answer

Thank you for your phone call. I am sorry to hear that ab80261 did not work as expected. Unfortunately, since the antibody was purchased over 1 year ago and our guarantee is valid for 6 months after the purchase, I am not able to offer a free of charge replacement, credit or refund in this case. However, I am happy to send you a testing discount code for trying ab70598 in human samples. DISCOUNT CODE: xxx Expiration date: xxx I am very pleased to hear you would like to accept our offer and test ab70598 in human. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview for human and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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