SOX4 一次抗体 製品一覧
製品の詳細Rabbit polyclonal to SOX4
アプリケーション適用あり: IHC-P, ICC/IF, WBmore details
交差が予測される動物種: Mouse, Rat, Rabbit, Goat, Horse, Chicken, Guinea pig, Cow, Dog, Drosophila melanogaster, Zebrafish
Synthetic peptide corresponding to Human SOX4 (N terminal). Immunogen located within the region from aa 38 and 87.
Database link: Q06945
- WB: HeLa whole cell lysate (ab150035). ICC/IF: SKNSH cells. IHC-P: Human testis tissue.
保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
バッファーPreservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS
Concentration information loading...
精製度Immunogen affinity purified
Our Abpromise guarantee covers the use of ab80261 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 µg/ml.|
|ICC/IF||Use a concentration of 1 µg/ml.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
機能Transcriptional activator that binds with high affinity to the T-cell enhancer motif 5'-AACAAAG-3' motif.
組織特異性Testis, brain, and heart.
配列類似性Contains 1 HMG box DNA-binding domain.
- Information by UniProt
- Ecotropic viral integration site 16 antibody
- EVI 16 antibody
- EVI16 antibody
Anti-SOX4 antibody (ab80261) at 1 µg/ml + HeLa cell lysate at 10 µg
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 47 kDa
Observed band size: 47 kDa
Additional bands at: 75 kDa. We are unsure as to the identity of these extra bands.
Gel concentration 12%.
ab80261 at 5 µg/ml staining SOX4 in human testis tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
ICC/IF image of ab80261 stained SKNSH cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab80261, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
This product has been referenced in:
- Yang YN et al. miR-204 reverses temozolomide resistance and inhibits cancer initiating cells phenotypes by degrading FAP-a in glioblastoma. Oncol Lett 15:7563-7570 (2018). Read more (PubMed: 29725461) »
- Liu Q et al. UCA1 promotes cell proliferation and invasion and inhibits apoptosis through regulation of the miR129-SOX4 pathway in renal cell carcinoma. Onco Targets Ther 11:2475-2487 (2018). Read more (PubMed: 29760557) »