Application
Western blot
Sample
Human Cell lysate - whole cell (H1 hESCs and H1 hESC derived neural progenitors)
Loading amount
20 µg
Specification
H1 hESCs and H1 hESC derived neural progenitors
Gel Running Conditions
Reduced Denaturing (7% SDS PAGE)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 20°C
Other product details
Dilution
1/1000
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 4% BSA in 1X TBST overnight incubation
Secondary antibody
Name
Non-Abcam antibody was used: Mouse anti-rabbit Light chain specific Jackson Lab
Host species: Mouse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Mouse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/20000
Detection
Detection method
Standard ECL
Exposure
30 second(s)
Bands
Specific: 50 kDa Non-specific: 190, 120, 105, 39 kDa
Positive control
Lane 2 = H1 Human embryonic stem cell (hESC) derived neural progenitor stem cells (NPSCs)
Negative control
Lane 1 = H1 hESCs
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Patrick Ovando Roche
Verified customer
投稿 Nov 19 2012