Application
Western blot
Sample
Human Cell lysate - whole cell (breast cancer cell lines)
Loading amount
50 µg
Specification
breast cancer cell lines
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Dilution
1/1000
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: TBS 1x - Tween 0.05% - milk 5%
Secondary antibody
Name
Non-Abcam antibody was used: DAKO P0260 anti-mouse secondary antibody
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/3000
Detection
Detection method
ECL
Exposure
6 minute(s) and 0 second(s)
Bands
Specific: 150 kDa
Positive control
all
Negative control
none
Additional data
Additional Notes
figure legend: 1-BT-20 2-BT-474 3-HBL-100 4-HCC-1937 5-HME-1 6-Hs-578T 7-MCF-10A 8-MDA-MB 157 9-MDA-MB 231 10-MDA-MB 436 11-MDA-MB 453 12-MDA-MB 549 13-T47D
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Jul 18 2007