保存方法Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Store undiluted.
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA
Concentration information loading...
精製度Immunogen affinity purified
Our Abpromise guarantee covers the use of ab115900 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 52 kDa.|
|IHC-P||Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
機能Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD9 is a receptor-regulated SMAD (R-SMAD).
組織特異性Expressed in heart, brain, placenta, lung, skeletal muscle, prostate, testis, ovary and small intestine. Also expressed in fetal brain, lung and kidney.
関連疾患Pulmonary hypertension, primary, 2
配列類似性Belongs to the dwarfin/SMAD family.
Contains 1 MH1 (MAD homology 1) domain.
Contains 1 MH2 (MAD homology 2) domain.
翻訳後修飾Phosphorylated on serine by BMP (bone morphogenetic proteins) type 1 receptor kinase.
細胞内局在Cytoplasm. Nucleus. In the cytoplasm in the absence of ligand. Migration to the nucleus when complexed with SMAD4.
- Information by UniProt
- MAD homolog 9 antibody
- Madh6 antibody
- Mothers against decapentaplegic antibody
ab115900, at 5 µg/ml, staining SMAD9 in formalin fixed, paraffin embedded Human kidney by Immunohistochemistry. Developed using biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Anti-SMAD9 antibody (ab115900) at 0.3 µg/ml + Human skin lysate in RIPA buffer at 35 µg
Developed using the ECL technique.
Predicted band size: 52 kDa
Primary incubation was 1 hour.