Application
Western blot
Sample
Human Cell lysate - whole cell (U2OS)
Loading amount
100000 cells
Specification
U2OS
Treatment
RNAi
Gel Running Conditions
Reduced Denaturing (7,5 %)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Dilution
1/1000
Incubation time
14 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBS-Tween 0.1%- BSA 5%
Secondary antibody
Name
Non-Abcam antibody was used: Pierce (31462)
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/30000
Detection
Detection method
ECL
Exposure
10 minute(s) and 0 second(s)
Bands
Specific: 120 kDa
Positive control
RNAi against SIRT1
Negative control
RNAi against an unrelated protein
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Feb 06 2008