Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa cells)
Loading amount
50 µg
Specification
HeLa cells
Gel Running Conditions
Reduced Denaturing (4-12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Dilution
1/500
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C
Secondary antibody
Name
Non-Abcam antibody was used: goat anti-rabbit HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000
Detection
Detection method
ECL
Exposure
1 minute(s) and 30 second(s)
Bands
Specific: 140 kDa Non-specific: many bands kDa
Positive control
lane 1 = 25 ug lysate
lane 2 = 50 ug lysate
lane 2 = 50 ug lysate
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Jul 20 2012