Anti-SGLT1 抗体 (ab14686)
Key features and details
- Rabbit polyclonal to SGLT1
- Suitable for: WB, ELISA, Dot blot, IP
- Reacts with: Rat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-SGLT1 antibody
SGLT1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to SGLT1 -
由来種
Rabbit -
アプリケーション
適用あり: WB, ELISA, Dot blot, IPmore details
適用なし: IHC-P -
種交差性
交差種: Rat, Human -
免疫原
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ポジティブ・コントロール
- WB: Rat enterocytes, rat heart, and human skeletal muscle tissues lysates.
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特記事項
A blocking peptide for this product is available as ab190911.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.02% Sodium azide
Constituents: Tris glycine, 0.5% BSA, HEPES, 30% Glycerol -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
Purified on antigen sepharose affinity column -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab14686の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (6) |
1/500 - 1/1000. Detects a band of approximately 74 kDa (predicted molecular weight: 73 kDa).
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ELISA |
Use at an assay dependent concentration.
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Dot blot |
Use at an assay dependent concentration.
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|
IP |
Use at an assay dependent concentration.
|
特記事項 |
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WB
1/500 - 1/1000. Detects a band of approximately 74 kDa (predicted molecular weight: 73 kDa). |
ELISA
Use at an assay dependent concentration. |
Dot blot
Use at an assay dependent concentration. |
IP
Use at an assay dependent concentration. |
ターゲット情報
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機能
Actively transports glucose into cells by Na(+) cotransport with a Na(+) to glucose coupling ratio of 2:1. Efficient substrate transport in mammalian kidney is provided by the concerted action of a low affinity high capacity and a high affinity low capacity Na(+)/glucose cotransporter arranged in series along kidney proximal tubules. -
組織特異性
Expressed mainly in intestine and kidney. -
関連疾患
Congenital glucose/galactose malabsorption -
配列類似性
Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family. -
翻訳後修飾
N-glycosylation is not necessary for the cotransporter function. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 6523 Human
- Entrez Gene: 25552 Rat
- Omim: 182380 Human
- SwissProt: P13866 Human
- SwissProt: P53790 Rat
- Unigene: 1964 Human
- Unigene: 10224 Rat
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別名
- D22S675 antibody
- High affinity sodium glucose cotransporter 1 antibody
- High affinity sodium glucose cotransporter antibody
see all
画像
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All lanes : Anti-SGLT1 antibody (ab14686) at 1/500 dilution
Lane 1 : Rat enterocytes
Lane 2 : Rat heart tissue
Lane 3 : Human skeletal muscle tissue
Predicted band size: 73 kDaTissue extracts were made in Solobuffer with protease inhibitors. The samples were solubilized in SDS-PAGE sample buffer and reduced with 2.5% BME.
Total 40-50ug protein was applied per lane on 10% SDS PAGE gels.
The proteins were transferred to nitrocellulose F and blocked with 5% milk and blotted with 1:500 diluted ab14686.The image was obtained with high sensitivity ECL assay and Image was captured with a CCD cooled camera for 30 sec.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (61)
ab14686 は 61 報の論文で使用されています。
- Korfhage J et al. Canagliflozin Increases Intestinal Adenoma Burden in Female ApcMin/+ Mice. J Gerontol A Biol Sci Med Sci 77:215-220 (2022). PubMed: 34448851
- Hirai H et al. The sodium/glucose cotransporters as potential therapeutic targets for CF lung diseases revealed by human lung organoid swelling assay. Mol Ther Methods Clin Dev 24:11-19 (2022). PubMed: 34977268
- Abdulrahman N et al. Empagliflozin inhibits angiotensin II-induced hypertrophy in H9c2 cardiomyoblasts through inhibition of NHE1 expression. Mol Cell Biochem 477:1865-1872 (2022). PubMed: 35334035
- Zapater JL et al. Enterocyte HKDC1 Modulates Intestinal Glucose Absorption in Male Mice Fed a High-fat Diet. Endocrinology 163:N/A (2022). PubMed: 35435980
- Zhang Y et al. Comparative study on the gastrointestinal- and immune- regulation functions of Hedysari Radix Paeparata Cum Melle and Astragali Radix Praeparata cum Melle in rats with spleen-qi deficiency, based on fuzzy matter-element analysis. Pharm Biol 60:1237-1254 (2022). PubMed: 35763552