The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IP: Use at 2-5µg/mg of lysate.
WB: 1/2000 - 1/10000. Detects bands of approximately 130 and 150 kDa (predicted molecular weight: 105 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
SAPS2 is a regulatory subunit for the protein phosphatase-6 catalytic subunit (PPP6C). Protein phosphatase regulatory subunits, such as SAPS2, modulate the activity of protein phosphatase catalytic subunits by restricting substrate specificity, recruiting substrates, and determining the intracellular localization of the holoenzyme. It is ubiquitously expressed with strongest expression in the testis followed by liver, heart, kidney, brain and placenta. There are two named isoforms.
All lanes : Anti-SAPS2 antibody (ab72031) at 0.04 µg/ml
Lane 1 : Whole cell lysate from Hela cells at 50 µg Lane 2 : Whole cell lysate from Hela cells at 15 µg Lane 3 : Whole cell lysate from Hela cells at 5 µg Lane 4 : Whole cell lysate from 293T cells at 50 µg
Immunoprecipitation/ Western Blot of SAPS2.
Lane 1: ab72031 at 3µg/mg whole cell lysate.
Lane 2: Control IgG.
Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded.
ab72031 at 1 µg/ml was used for the Western blot detection step.
Chemiluminescence with an exposure time of 3 seconds.