Anti-S100 Calcium Binding Protein A13/S100A13 抗体 [EPR4510] (ab109252)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4510] to S100 Calcium Binding Protein A13/S100A13
- Suitable for: WB, IP, IHC-P, Flow Cyt (Intra), ICC/IF
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-S100 Calcium Binding Protein A13/S100A13 antibody [EPR4510]
S100 Calcium Binding Protein A13/S100A13 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR4510] to S100 Calcium Binding Protein A13/S100A13 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IP, IHC-P, Flow Cyt (Intra), ICC/IFmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- EB: HeLa, HEK293T, HUVEC and ECV304 cell lysates; Human thyroid and testis tissue lysates. IHC-P: Human brain and muscle tissues. ICC//IF: HUVEC cells Flow Cyt (intra): HUVEC cells IP: Human heart lysate.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR4510 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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KO cell lines
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab109252の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 11 kDa.
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IP |
1/10 - 1/100.
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IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Use of a HRP/AP polymerized antibody recommended. |
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Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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ICC/IF |
1/100 - 1/250.
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特記事項 |
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WB
1/1000 - 1/10000. Predicted molecular weight: 11 kDa. |
IP
1/10 - 1/100. |
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Use of a HRP/AP polymerized antibody recommended. |
Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
ICC/IF
1/100 - 1/250. |
ターゲット情報
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機能
Plays a role in the export of proteins that lack a signal peptide and are secreted by an alternative pathway. Binds two calcium ions per subunit. Binds one copper ion. Binding of one copper ion does not interfere with calcium binding. Required for the copper-dependent stress-induced export of IL1A and FGF1. The calcium-free protein binds to lipid vesicles containing phosphatidylserine, but not to vesicles containing phosphatidylcholine. -
組織特異性
Expressed in heart and skeletal muscle. -
配列類似性
Belongs to the S-100 family.
Contains 2 EF-hand domains. -
細胞内局在
Cytoplasm. Secreted. Secretion is mediated by exposure to stress and requires copper ions. - Information by UniProt
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参照データベース
- Entrez Gene: 6284 Human
- Omim: 601989 Human
- SwissProt: Q99584 Human
- Unigene: 516505 Human
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別名
- Protein S100 A13 antibody
- Protein S100-A13 antibody
- S100 calcium binding protein A13 antibody
see all
画像
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All lanes : Anti-S100 Calcium Binding Protein A13/S100A13 antibody [EPR4510] (ab109252) at 1/1000 dilution
Lane 1 : Wild-type HEK-293T cell lysate
Lane 2 : S100A13 knockout HEK-293T cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : Daudi cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 11 kDa
Observed band size: 13 kDa why is the actual band size different from the predicted?Lanes 1 - 4: Merged signal (red and green). Green - ab109252 observed at 13 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37 kDa.
ab109252 was shown to react with S100 Calcium Binding Protein A13/S100A13 in wild-type HEK-293T cells in Western blot with loss of signal observed in S100A13 knockout cell line ab266065 (S100A13 knockout cell lysate ab258183). Wild-type HEK-293T and S100A13 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab109252 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.
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Immunocytochemistry/ Immunofluorescence analysis of HUVEC (human umbilical vein endothelial cell) cells labeling S100 Calcium Binding Protein A13/S100A13 with purified ab109252 at 1/100 dilution (10 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL) was used as the secondary antibody only control.
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ab109252, at a 1/250 dilution, staining S100 Calcium Binding Protein A13/S100A13 in Human brain, by Immunohistochemistry, Paraffin-embedded tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Intracellular Flow Cytometry analysis of HUVEC (Human umbilical vein endothelial cell) cells labeling S100 Calcium Binding Protein A13/S100A13 with purified ab109252 at 1/100 dilution (10 µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cells without incubation with primary antibody and secondary antibody (Blue).
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All lanes : Anti-S100 Calcium Binding Protein A13/S100A13 antibody [EPR4510] (ab109252) at 1/1000 dilution
Lane 1 : Human thyroid tissue lysate
Lane 2 : Human testis tissue lysate
Lane 3 : HUVEC lysate
Lane 4 : ECV304 lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 11 kDa -
Purified ab109252 at 1/50 dilution (2µg) immunoprecipitating S100 Calcium Binding Protein A13/S100A13 in Human heart lysate.
Lane 1 (input): Human heart lysate 10µg
Lane 2 (+): ab109252 + Human heart lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab109252 in Human heart lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 11 kDa -
ab109252, at a 1/250 dilution, staining S100 Calcium Binding Protein A13/S100A13 in Human muscle, by Immunohistochemistry, Paraffin-embedded tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab109252 は 1 報の論文で使用されています。
- Wang X et al. Disulfiram Exerts Antifibrotic and Anti-Inflammatory Therapeutic Effects on Perimysial Orbital Fibroblasts in Graves' Orbitopathy. Int J Mol Sci 23:N/A (2022). PubMed: 35563653