Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 10µg/mL · Temperature: RT°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris EDTA pH 9
Sample
Dog Tissue sections (MDCK pellets in paraffin)
Specification
MDCK pellets in paraffin
Permeabilization
No
Fixative
Formaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Diluent: TBS-T
Dilution
1/100
Secondary antibody
Name
Non-Abcam antibody was used: Leyca Powervision anti Rb
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Additional data
Additional Notes
We can't be sure that antibody works on dog samples, because the different level of staining in a pellet of an unique cell line...
Moreover, nuclear staining should not be observed in any case.
Abcam response
Thank you for your valuable feedback. We have not previously tested this antibody in dog and therefore it may not be suitable for use in this species. We welcome other abreviews to determine this.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Oct 13 2014