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Our customer is going to purchase Adiponectin protein for WB(human sample).
She found different form of this protein such as his tag, tagged and active form on the website.
So she would like to know which one is a good choice.
Asked on May 30 2012
Thank you for contacting us.
I would like to suggest not to use an active protein as this is not unnecessary in my eyes as a positive control (it's a bit a waste of money), when a tagged or non-tagged protein will do.
A tagged protein can be a positive control in two ways, on way to check if the anti- Adiponectinantibody is working,plus if she is tagging her protein, she can checkif theprotein tagging was successful with an anti-tag antibody.
However, if she just needs a positive control, a simply inactive, non-tagged protein will do just fine.
It really depends on what your customer prefers.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
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Abcam Scientific Support
Answered on May 30 2012