Recombinant human PDK3 protein (ab125606)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 90% Densitometry
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: WB, Functional Studies, SDS-PAGE
製品の詳細
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製品名
Recombinant human PDK3 protein -
生理活性
The specific activity of ab125606 was determined to be 12 nmol/mg/min.
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精製度
> 90 % Densitometry.
Purity determined to be >90% by densitometry. Affinity purified. Purity is lot specific. Please contact our technical Support team for details. -
発現系
Baculovirus infected Sf9 cells -
アクセッション番号
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タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
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生物種
Human -
予測される分子量
69 kDa including tags -
領域
1 to 406 -
タグ
GST tag N-Terminus
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関連製品
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Substrate reagent
特性
Our Abpromise guarantee covers the use of ab125606 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
Western blot
Functional Studies
SDS-PAGE
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製品の状態
Liquid -
備考
ab125602 (Human Pyruvate Dehydrogenase E1-alpha subunit full length protein) can be utilized as a substrate for assessing Kinase activity
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Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.31% Glutathione, 0.002% PMSF, 0.004% DTT, 0.79% Tris HCl, 0.003% EDTA, 25% Glycerol (glycerin, glycerine), 0.88% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
関連情報
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別名
- [Pyruvate dehydrogenase [lipoamide] kinase isozyme 3 mitochondrial
- [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 3
- CMTX6
see all -
機能
Inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism. -
組織特異性
Found almost exclusively in heart and skeletal muscle. -
配列類似性
Belongs to the PDK/BCKDK protein kinase family.
Contains 1 histidine kinase domain. -
細胞内局在
Mitochondrion matrix. - Information by UniProt
画像
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab125606 は論文での使用が確認できていません。