Recombinant human MAP4K1/HPK1 protein (ab107953)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 75% SDS-PAGE
- Active: Yes
- Suitable for: SDS-PAGE, WB, Functional Studies
製品の詳細
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製品名
Recombinant human MAP4K1/HPK1 protein
MAP4K1/HPK1 タンパク質・ペプチド 製品一覧 -
生理活性
The Specific activity of ab107953 was determined to be 125 nmol/min/mg.
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精製度
> 75 % SDS-PAGE.
Purity was determined to be >75% by densitometry. Affinity purified. -
発現系
Baculovirus infected Sf9 cells -
アクセッション番号
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タンパク質長
Protein fragment -
Animal free
No -
由来
Recombinant -
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生物種
Human -
予測される分子量
65 kDa including tags -
領域
1 to 346
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関連製品
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Related Products
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Substrate reagent
特性
Our Abpromise guarantee covers the use of ab107953 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
SDS-PAGE
Western blot
Functional Studies
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製品の状態
Liquid -
備考
ab64311 (Myelin Basic Protein protein) can be utilized as a substrate for assessing kinase activity
This product was previously labelled as MAP4K1
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Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.307% Glutathione, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol (glycerin, glycerine), 0.87% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
関連情報
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別名
- Hematopoietic progenitor kinase
- Hematopoietic progenitor kinase 1
- HPK1
see all -
機能
May play a role in the response to environmental stress. Appears to act upstream of the JUN N-terminal pathway. May play a role in hematopoietic lineage decisions and growth regulation. -
組織特異性
Expressed primarily in hematopoietic organs, including bone marrow, spleen and thymus. Also expressed at very low levels in lung, kidney, mammary glands and small intestine. -
配列類似性
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
Contains 1 CNH domain.
Contains 1 protein kinase domain. - Information by UniProt
画像
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The specific activity of MAP4K1/HPK1 (ab107953) was determined to be 110 nmol/min/mg as per activity assay protocol and was equivalent to 144 nmol/min/mg as per radiometric assay
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SDS PAGE analysis of ab107953
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SDS PAGE analysis of ab107953
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SDS-PAGE showing ab107953 at approximately 65kDa.
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Kinase Assay demonstrating specific activity of ab107953.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab107953 は論文での使用が確認できていません。