製品の概要

製品の詳細

  • 由来
    Recombinant
  • 由来
    Escherichia coli
  • アミノ酸配列
    • アクセッション番号
    • 生物種
      Human
    • 分子量
      60 kDa including tags
    • 領域
      1 to 310

特性

Our Abpromise guarantee covers the use of ab101133 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • アプリケーション

    Western blot

    SDS-PAGE

  • 精製度
    > 95 % SDS-PAGE.
    The purity was determined to be >95% by densitometry
  • 製品の状態
    Liquid
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.307% Glutathione, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol, 0.87% Sodium chloride

関連情報

  • 別名
    • NAD dependent deacetylase sirtuin 5
    • NAD dependent lysine demalonylase and desuccinylase sirtuin 5 mitochondrial
    • NAD dependent protein deacylase sirtuin 5 mitochondrial
    • NAD-dependent protein deacylase sirtuin-5, mitochondrial
    • Regulatory protein SIR2 homolog 5
    • Silent mating type information regulation 2 S.cerevisiae homolog 5
    • Sir2 like 5
    • SIR2-like protein 5
    • SIR2L5
    • SIR5_HUMAN
    • Sirt5
    • Sirtuin 5
    • Sirtuin type 5
    see all
  • 機能
    NAD-dependent lysine demalonylase, desuccinylase and deglutarylase that specifically removes malonyl, succinyl and glutaryl groups on target proteins (PubMed:21908771, PubMed:22076378, PubMed:24703693). Activates CPS1 and contributes to the regulation of blood ammonia levels during prolonged fasting: acts by mediating desuccinylation and deglutarylation of CPS1, thereby increasing CPS1 activity in response to elevated NAD levels during fasting (PubMed:22076378, PubMed:24703693). Activates SOD1 by mediating its desuccinylation, leading to reduced reactive oxygen species (PubMed:24140062). Modulates ketogenesis through the desuccinylation and activation of HMGCS2 (By similarity). Has weak NAD-dependent protein deacetylase activity; however this activity may not be physiologically relevant in vivo. Can deacetylate cytochrome c (CYCS) and a number of other proteins in vitro such as UOX.
  • 組織特異性
    Widely expressed.
  • 配列類似性
    Belongs to the sirtuin family. Class III subfamily.
    Contains 1 deacetylase sirtuin-type domain.
  • ドメイン
    In contrast to class I sirtuins, class III sirtuins have only weak deacetylase activity. Difference in substrate specificity is probably due to a larger hydrophobic pocket with 2 residues (Tyr-102 and Arg-105) that bind to malonylated and succinylated substrates and define the specificity (PubMed:22076378).
  • 細胞内局在
    Mitochondrion; Cytoplasm. Mitochondrion and Mitochondrion matrix. Mitochondrion intermembrane space. Cytoplasm, cytosol. Nucleus. Mainly mitochondrial. Also present extramitochondrially: a fraction is present in the cytosol and very small amounts are also detected in the nucleus.
  • Information by UniProt

画像

  • SDS-PAGE showing ab101133 at approximately 60kDa.

参考文献

ab101133 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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