Anti-Radixin/RDX 抗体 [EP1862Y] (ab52495)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1862Y] to Radixin/RDX
- Suitable for: Flow Cyt (Intra), ICC/IF, WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Radixin/RDX antibody [EP1862Y]
Radixin/RDX 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EP1862Y] to Radixin/RDX -
由来種
Rabbit -
アプリケーション
適用あり: Flow Cyt (Intra), ICC/IF, WB, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat -
免疫原
Synthetic peptide within Human Radixin/RDX aa 350-450 (N terminal). The exact sequence is proprietary.
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ポジティブ・コントロール
- WB: HeLa cell lysate. IHC-P: Paraffin embedded human ovarian carcinoma. Flow Cyt (intra): HeLa cells. ICC/IF: Mcf7 cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EP1862Y -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab52495の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Flow Cyt (Intra) |
1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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ICC/IF | (2) |
Use a concentration of 5 µg/ml.
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WB | (2) |
1/1000 - 1/10000. Detects a band of approximately 80 kDa (predicted molecular weight: 80 kDa).
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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特記事項 |
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Flow Cyt (Intra)
1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
ICC/IF
Use a concentration of 5 µg/ml. |
WB
1/1000 - 1/10000. Detects a band of approximately 80 kDa (predicted molecular weight: 80 kDa). |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Probably plays a crucial role in the binding of the barbed end of actin filaments to the plasma membrane. -
関連疾患
Defects in RDX are the cause of deafness autosomal recessive type 24 (DFNB24) [MIM:611022]. DFNB24 is a form of sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information. -
配列類似性
Contains 1 FERM domain. -
ドメイン
The N-terminal domain interacts with the C-terminal domain of LAYN. An interdomain interaction between its N-terminal and C-terminal domains inhibits its ablilty to bind LAYN. In the presence of acidic phospholipids, the interdomain interaction is inhibited and this enhances binding to LAYN. -
翻訳後修飾
Phosphorylated by tyrosine-protein kinases. -
細胞内局在
Cell membrane. Cytoplasm > cytoskeleton. Cleavage furrow. Highly concentrated in the undercoat of the cell-to-cell adherens junction and the cleavage furrow in the interphase and mitotic phase, respectively. - Information by UniProt
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参照データベース
- Entrez Gene: 5962 Human
- Entrez Gene: 19684 Mouse
- Entrez Gene: 315655 Rat
- Omim: 179410 Human
- SwissProt: P35241 Human
- SwissProt: P26043 Mouse
- Unigene: 263671 Human
- Unigene: 592679 Human
see all -
別名
- CB567 antibody
- CG12537 antibody
- DFNB24 antibody
see all
画像
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Anti-Radixin/RDX antibody [EP1862Y] (ab52495) at 1/1000 dilution + HeLa cell lysate at 10 µg
Secondary
HRP labeled goat anti-rabbit at 1/2000 dilution
Predicted band size: 80 kDa
Observed band size: 80 kDa -
Immunohistochemical staining of paraffin-embedded human ovarian carcinoma using ab52495 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF image of ab52495 stained Mcf7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab52495, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Overlay histogram showing HeLa cells stained with ab52495 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab52495, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (22)
ab52495 は 22 報の論文で使用されています。
- Seker U et al. Effects of black cumin seed oil on oxidative stress and expression of membrane-cytoskeleton linker proteins, radixin, and moesin in streptozotocin-induced diabetic rat liver. Hepatol Forum 3:21-26 (2022). PubMed: 35782372
- Zhang Y et al. Oocyte-derived microvilli control female fertility by optimizing ovarian follicle selection in mice. Nat Commun 12:2523 (2021). PubMed: 33953177
- Zhang L et al. STK10 knockout inhibits cell migration and promotes cell proliferation via modulating the activity of ERM and p38 MAPK in prostate cancer cells. Exp Ther Med 22:851 (2021). PubMed: 34149897
- Zhang L et al. Knockout of STK10 promotes the migration and invasion of cervical cancer cells. Transl Cancer Res 9:7079-7090 (2020). PubMed: 35117313
- Zhang N et al. LncRNA linc01116 prometes glioma cell migration and invasion by modulation of radixin targeted by miR-31. Int J Clin Exp Pathol 12:1078-1086 (2019). PubMed: 31933922