Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 0.01M citric acid/citrate sodium
Sample
Mouse Tissue sections (mouse testes)
Specification
mouse testes
Permeabilization
No
Fixative
4% PFA
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: over night
Dilution
1/200
Secondary antibody
Name
Non-Abcam antibody was used: Donkey Anti-mouse IgG (H+L)715-095-150 Jackson
Host species: Donkey
Clonality: Polyclonal
Conjugation: Fluorescein
Host species: Donkey
Clonality: Polyclonal
Conjugation: Fluorescein
Dilution
1/100
Additional data
Abcam response
Thank you for your valuable data. It appears that under these experimental conditions, ab1837 produces a staining pattern similar to that observed with ab46981 in HEK293 cells.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 May 29 2013