Anti-RNF128/GRAIL 抗体 (ab72533)
Key features and details
- Rabbit polyclonal to RNF128/GRAIL
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-RNF128/GRAIL antibody
RNF128/GRAIL 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to RNF128/GRAIL -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Mouse, Rat, Human
交差が予測される動物種: Chicken, Cow, Opossum, Orangutan -
免疫原
Synthetic peptide corresponding to Human RNF128/GRAIL aa 227-253.
Sequence:IFYSARRLRNARAQSRKQRQLKADAKK
Database link: NP_919445.1 -
ポジティブ・コントロール
- Human, mouse and rat spleen lysates. Human brain tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: 0.2% BSA, PBS -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab72533の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 47 kDa).
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IHC-P |
Use a concentration of 5 - 10 µg/ml.
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特記事項 |
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WB
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 47 kDa). |
IHC-P
Use a concentration of 5 - 10 µg/ml. |
ターゲット情報
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機能
E3 ubiquitin-protein ligase that catalyzes polyubiquitin chains. Functions as an inhibitor of cytokine gene transcription. Inhibits IL2 and IL4 transcription and this activity is likely to be mediated by E3 ligase activity. Plays an important role in the induction of the anergic phenotype. Functions in the patterning of the dorsal ectoderm; sensitizes ectoderm to respond to neural-inducing signals. -
パスウェイ
Protein modification; protein ubiquitination. -
配列類似性
Contains 1 PA (protease associated) domain.
Contains 1 RING-type zinc finger. -
ドメイン
Binding to E2 ubiquitin-conjugating enzyme requires an intact RING finger domain. -
翻訳後修飾
Auto-ubiquitinated. Controls the development of T cell clonal anergy by ubiquitination. -
細胞内局在
Endomembrane system. Cytoplasm > perinuclear region. Localized in an asymmetric perinuclear punctate manner. Localizes to the internal pool of the transferrin recycling endosomal pathway. Partially colocalized with the endoplasmic reticulum resident HSPA5, with Golgi resident STX5, and with the late endosomal GTPase RAB7. - Information by UniProt
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参照データベース
- Entrez Gene: 422382 Chicken
- Entrez Gene: 535869 Cow
- Entrez Gene: 79589 Human
- Entrez Gene: 66889 Mouse
- Entrez Gene: 315911 Rat
- Omim: 300439 Human
- SwissProt: Q29RU0 Cow
- SwissProt: Q8TEB7 Human
see all -
別名
- E3 ubiquitin protein ligase RNF128 antibody
- E3 ubiquitin-protein ligase RNF128 antibody
- FLJ23516 antibody
see all
画像
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All lanes : Anti-RNF128/GRAIL antibody (ab72533) at 1 µg/ml
Lane 1 : human spleen lysate
Lane 2 : human spleen lysate with immunising peptide
Lane 3 : mouse spleen lysate
Lane 4 : rat spleen lysate
Predicted band size: 47 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?
Additional bands at: 25 kDa. We are unsure as to the identity of these extra bands.Endogenous RNF128 / GRAIL is reported to be detected at approximately 66 kD
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Immunohistochemical staining of paraffin-embedded normal human brain tissue using ab72533 at 5 ug/ml (Panel A) and control rabbit Ig at 5 ug/ml (Panel B).
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (1)
ab72533 は 1 報の論文で使用されています。
- Bai XS et al. RNF128 Promotes Malignant Behaviors via EGFR/MEK/ERK Pathway in Hepatocellular Carcinoma. Onco Targets Ther 13:10129-10141 (2020). PubMed: 33116595