Application
Immunohistochemistry (Frozen sections)
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 24°C
Sample
Rat Tissue sections (pituitary gland of mRFP1 gene transgenic rat)
Specification
pituitary gland of mRFP1 gene transgenic rat
Permeabilization
Yes - Triton-X
Fixative
Paraformaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1%BSA, 1%Donkey Normal Serum in PBS
Dilution
1/100
Secondary antibody
Name
Non-Abcam antibody was used: Histofine Simple Stain Rat MAX-PO(R)
Host species: Goat
Clonality: Polyclonal
Conjugation: HRP polymer
Host species: Goat
Clonality: Polyclonal
Conjugation: HRP polymer
Additional data
Additional Notes
This antibody can identify mRFP1!
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Oct 30 2013