Application
Western blot
Loading amount
10 µg
Gel Running Conditions
Non-reduced Non-Denaturing (Native)
Sample
Monkey Cell lysate - other (cos7 cell)
Specification
cos7 cell
Treatment
overexpress RFP construct
Blocking step
(agent) for 1 hour(s) and 0 minute(s) · Concentration: 8% · Temperature: 22°C
Other product details
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/500
Secondary antibody
Name
Non-Abcam antibody was used: HRP anti rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/3000
Detection
Detection method
ECL
Bands
Specific: 27 kDa Non-specific: ~18 kDa
Exposure
1 minute(s) and 0 second(s)
Negative control
GFP construct
Positive control
RFP transfected constrcut
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投稿 Oct 01 2013