Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Human Umbilical Vein Endothelial Cells)
Permeabilization
Yes - 0.5%TRITON x-100 for 20mins
Specification
Human Umbilical Vein Endothelial Cells
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 37°C
Fixative
Paraformaldehyde
Other product details
Incubation time
10 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/200
Secondary antibody
Dilution
1/1000
Name
Non-Abcam antibody was used: Donkey anti-Rabbit IgG, Alexa Fluor 488
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor 488
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor 488
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. 振洋 余
Verified customer
投稿 Mar 24 2017