Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rabbit Tissue sections (intestinum)
Specification
intestinum
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated
Permeabilization
No
Blocking step
Blocking Solution Zytomed as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Other product details
Dilution
1/150
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Antibody Diluent, Fa.Zytomed
Secondary antibody
Name
Non-Abcam antibody was used: ABC
Clonality: Polyclonal
Conjugation: Biotin
Clonality: Polyclonal
Conjugation: Biotin
Additional data
Additional Notes
Broad spectrum streptavidin-biotin system used for detection. Able to detect primary antibodies raised in mouse, rabbit, rat and guinea pig.
Abcam response
Thank you for submitting this review. We have as yet not received any other reports with this antibody being used with rabbit samples. As the homology is 90% between the rabbit protein and the human protein against which this antibody was raised, the antibody may well cross react. We would welcome any reports from customers who have tried the antibody with rabbit samples, on in IHC.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Mar 01 2013