Anti-Pumilio 1 抗体 [EPR3795] (ab92545)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3795] to Pumilio 1
- Suitable for: ICC/IF, WB, IHC-P, Flow Cyt (Intra)
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Pumilio 1 antibody [EPR3795]
Pumilio 1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR3795] to Pumilio 1 -
由来種
Rabbit -
特異性
This antibody is predicted to not cross react with Pumilio 2. -
アプリケーション
適用あり: ICC/IF, WB, IHC-P, Flow Cyt (Intra)more details
適用なし: IP -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide. within Human Pumilio 1 aa 150-250. The exact sequence is proprietary.
Database link: Q14671 -
ポジティブ・コントロール
- WB: HeLa, Mouse cerebellum, rat brain and 293T cell lysates IHC-P: Human fetal kidney tissue, human thyroid carcinoma. ICC/IF: HeLa cells
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR3795 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab92545の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
ICC/IF |
1/500.
For unpurified use at 1/100 - 1/250. |
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WB |
1/1000 - 1/5000. Predicted molecular weight: 126 kDa.
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IHC-P |
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
This antibody may not be suitable for IHC with mouse or rat samples. |
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Flow Cyt (Intra) |
1/20.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. For unpurified use at 1/100. |
特記事項 |
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ICC/IF
1/500. For unpurified use at 1/100 - 1/250. |
WB
1/1000 - 1/5000. Predicted molecular weight: 126 kDa. |
IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. This antibody may not be suitable for IHC with mouse or rat samples. |
Flow Cyt (Intra)
1/20. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. For unpurified use at 1/100. |
ターゲット情報
- Information by UniProt
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参照データベース
- Entrez Gene: 9698 Human
- Entrez Gene: 80912 Mouse
- Entrez Gene: 362609 Rat
- Omim: 607204 Human
- SwissProt: Q14671 Human
- SwissProt: Q80U78 Mouse
- Unigene: 281707 Human
- Unigene: 440206 Mouse
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製品の状態
Cytoplasm -
別名
- HsPUM antibody
- KIAA0099 antibody
- PUM 1 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human thyroid carcinoma tissue sections labeling Pumilio 1 with Purified ab92545 at 1:100 dilution (1.18 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.
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Intracellular Flow Cytometry analysis of HEK-293 (Human embryonic kidney epithelial cell) cells labeling Pumilio 1 with purified ab92545 at 1/20 dilution (10 ug/ml) (red). Cells were fixed with 4% Paraformaldehyde and permeabilized with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluorr® 488) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
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Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Pumilio 1 with purified ab92545 at 1:500 dilution (0.2μg/ml). Cells were fixed in 100% Methanol. ab150077 Goat anti rabbit IgG(Alexa Fluor® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
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All lanes : Anti-Pumilio 1 antibody [EPR3795] (ab92545) at 1/1000 dilution (unpurified)
Lane 1 : HeLa cell lysate
Lane 2 : 293T cell lysates
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 126 kDa -
Immunohistochemical analysis of Pumilio 1 in paraffin embedded Human fetal kidney tissue using an unpurified ab92545 at a 1/100 dilution.
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All lanes : Anti-Pumilio 1 antibody [EPR3795] (ab92545) at 1/1000 dilution (purified)
Lane 1 : Mouse cerebellum tissue lysate
Lane 2 : Rat brain tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 126 kDaBlocking and diluting buffer: 5% NFDM/TBST
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All lanes : Anti-Pumilio 1 antibody [EPR3795] (ab92545) at 1/5000 dilution (purified)
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 126 kDaBlocking and diluting buffer: 5% NFDM/TBST
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Immunofluorescent staining of Pumilio 1 in HeLa cells using an unpurified ab92545 at a 1/100 dilution.
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Overlay histogram showing HEK293 cells stained with an unpurified ab92545 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab92545, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HEK293 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (10)
ab92545 は 10 報の論文で使用されています。
- Gong Y et al. PUMILIO proteins promote colorectal cancer growth via suppressing p21. Nat Commun 13:1627 (2022). PubMed: 35338151
- Ledet RJ et al. Identification of PIM1 substrates reveals a role for NDRG1 phosphorylation in prostate cancer cellular migration and invasion. Commun Biol 4:36 (2021). PubMed: 33398037
- Kopp F et al. PUMILIO hyperactivity drives premature aging of Norad-deficient mice. Elife 8:N/A (2019). PubMed: 30735131
- Elguindy MM et al. PUMILIO, but not RBMX, binding is required for regulation of genomic stability by noncoding RNA NORAD. Elife 8:N/A (2019). PubMed: 31343408
- Lin K et al. Mammalian Pum1 and Pum2 Control Body Size via Translational Regulation of the Cell Cycle Inhibitor Cdkn1b. Cell Rep 26:2434-2450.e6 (2019). PubMed: 30811992
- Lin K et al. Essential requirement of mammalian Pumilio family in embryonic development. Mol Biol Cell 29:2922-2932 (2018). PubMed: 30256721
- Muench MO et al. The human chorion contains definitive hematopoietic stem cells from the fifteenth week of gestation. Development 144:1399-1411 (2017). PubMed: 28255007
- Zhang M et al. Post-transcriptional regulation of mouse neurogenesis by Pumilio proteins. Genes Dev N/A:N/A (2017). PubMed: 28794184
- Hubstenberger A et al. P-Body Purification Reveals the Condensation of Repressed mRNA Regulons. Mol Cell 68:144-157.e5 (2017). PubMed: 28965817
- Mak W et al. An Important Role of Pumilio 1 in Regulating the Development of the Mammalian Female Germline. Biol Reprod 94:134 (2016). PubMed: 27170441