Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (liver, mammary gland)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer ph 6.0
Permeabilization
No
Specification
liver, mammary gland
Blocking step
protein block as blocking agent for 10 minute(s) · Concentration: 100% · Temperature: rt°C
Fixative
Formaldehyde
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Diluent: antibody diluent
Secondary antibody
Name
Non-Abcam antibody was used: Envision+HRP system mouse
Host species: Mouse
Clonality: Polyclonal
Conjugation: Streptavidin
Host species: Mouse
Clonality: Polyclonal
Conjugation: Streptavidin
Additional data
Additional Notes
avidin blocking : 15 minutes, biotin blocking 15 minutes room temperature.
Abcam response
Our Scientific Support team is currently investigating this negative Abreview, and we will resolve the issue in accordance with our Abpromise.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MS. Francine Deslippe
Verified customer
投稿 Apr 10 2013