Application
Western blot
Sample
Rat Tissue lysate - whole (Retina)
Loading amount
30 µg
Specification
Retina
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Other product details
Dilution
1/1000
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% BSA
Secondary antibody
Name
Non-Abcam antibody was used: Anti-mouse
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/3000
Detection
Detection method
ECL
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 85, 50 kDa Non-specific: 180 kDa
Positive control
Human Prekallikrein
Additional data
PubMed ID
17259996: View
Additional Notes
The antibody block the conversion of prekallikrein to kallikrein in vivo in rat. (Gao, et al. NATURE MEDICINE, 200713(2)181-188
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Dec 31 2008